Items 1-12 of 100 Results
-
Cells in Action: Double mitosis of Human Umbilical Vein Endothelial Cells (HUVECs)
Images of cells undergoing mitosis are mind-blowing, and non more beautiful than those of our Human Umbilical Vein Endothelial Cells (HUVECs) captured by Nanolive's 3D Cell Explorer. On the right hand side, the cell seems to enter mitosis but the chromatids don't complete segregation and the cell returns to interphase without dividing. Exiting mitosis is controlled by proteolysis and cyclin dependent kinases (CDKs). Mitosis regulatory machinery sometimes detects errors and forces a return to interphase, as seen here. This kind of research helps develop understanding of the cell cycle, which can be used in myriad applications including cancer research. -
Cells in Action: membrane protrusions in HUVECs (Human Umbilical Vein Endothelial Cells)
During angiogenesis, cell-matrix interactions are crucial. The cell matrix structures are involved in migration, invasion and survival of cells throughout the angiogenic process. In this video we can see filopodia and lamellipodia in great detail, thanks to Nanolive imaging's ability to view membrane deformations. These structures are essential for migration, cell-cell interactions, sensing of the cell environment and more. While filopodia appear as long, thin protrusion-like structures that emerge from the cellular membrane, lamellipodia are broad, sheet-shaped structures containing thin and short interconnected actin filaments. -
Cells in Action: Mitosis in Human Mesenchymal Stem Cells (MSCs)
Watch the process of cell division in PromoCell's human mesenchymal stem cells! These images were captured using Nanolive’s non-invasive technology, which allows us to access the dynamics of biological processes, such as mitosis, with unprecedented resolution. -
Cells in action: Differentiation of human mesenchymal stem cells into neurons
Cells in action: Differentiation of human mesenchymal stem cells into neurons In this video we can see the differentiation of PromoCell umbilical cord mesenchymal stem cells into neurons. Excitingly, to our knowledge this is one of the first high-resolution, long-term, live cell time-lapses showing this process. These cells were grown for 13 days in PromoCell complete mesenchymal stem cell neurogenic differentiation medium prior to capture of this film. Images were captured every 30 seconds over a 20-hour period using NanoLive’s 3D Cell Explorer. -
Cells in Action: Human Primary Keratinocytes at the stratum granulosum
Keratinocytes at the stratum granulosum contain two characteristic structures that are visible in this high resolution footage by Nanolive - the lamellar bodies and keratohyalin granules. While keratohyalin granules contain proteins involved in the aggregation of keratin filaments and in the formation of the cell envelope, lamellar bodies contain lipids and fuse with the plasma membrane in order to secrete the content into the extracellular space. -
High-yield isolation of HUVECs in Endothelial Cell Growth Medium 2
Here we show a procedure for a high-yield isolation of primary HUVECs in PromoCell’s Endothelial Cell Growth Medium 2 (ECGM2) and the comparison with a competitor medium. -
Thawing frozen cells – detailed protocol for human primary cells
Thawing cells correctly is absolutely necessary when it comes to successfully starting reliable cell cultures. Find out how to thaw frozen primary cells properly in our detailed video protocol. -
HLA-Typed Primary Cells: The advantages of working with HLA in drug development research
For those working in drug development, human leukocyte antigens or HLA are a key element to keep in mind as an essential regulator of the immune system. Watch our latest explainer video to understand the benefits of working with HLA in your pre-clinical research. -
Cell Growth, Cell Death and Aging of Cells
Dr. Rüdiger Arnold, one of our PromoCell Academy lecturers, explains the basics of cell growth, cell quantification and apoptosis as well as best practices. -
Attachment and Detachment of Cells
Dr. Rüdiger Arnold explains the basics of attachment and detachment of cells. -
Mycoplasma Contamination in Cell Culture
Mycoplasma is the nightmare of every scientist working with Cell Culture. Mycoplasma contamination is threatening academic labs to biopharmaceutical production facilities. Dr. Rüdiger Arnold explains why best practice in detection, elimination and avoiding mycoplasma are necessary for your cell culture. -
Primary cells in respiratory research – webinar
Learn about the benefits of primary cells as model systems in respiratory research and how a 3D model at the Air-Liquid Interface can be set up and used.